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HRbio™ Taq DNA Polymerase DNA
Price：
￥150
Cargo Number：
HRF0011
Specification：
1000U 5×1000U
Specification and quality control：
Product Details
Product Specification
Product Information
Product Name | Product Number | Specification | Price (Yuan) |
HRbio™ Taq DNA Polymerase | HRF0011 | 1000 U | 150.00 |
HRbio™ Taq DNA Polymerase | HRF0012 | 5×1000 U | 700.00 |
Product Description
HRbio™ Taq DNA Polymerase is isolated and purified from E. coli cloned with Thermu aquaticus DNA Polymerase gene after induction, with a molecular weight of 94 kD. Taq DNA Polymerase has 5'→3' DNA polymerase activity and 5'→3' exonuclease activity, but no 3'→5' exonuclease activity. In the PCR reaction, the extension rate of Taq DNA Polymerase is 1-2 kb/min, and the product has A at the 3' end, which can be directly used for T/A vector cloning.
Product composition
serial number | Components | Product Number/Specification | |
HRF0011 (1000 U) | HRF0012 (5 × 1000 U) | ||
HRF001-A | 10×Taq Buffer (with Mg 2+ ) | 2×1.5 mL | 10×1.5 mL |
HRF001-B | HRbio™ Taq DNA Polymerase (5U/ μL ) | 200 μL | 5×200 μL |
Application
It is commonly used for PCR amplification of DNA fragments, genotype identification, colony PCR and other conventional PCR, DNA labeling, primer extension, sequence determination, blunt end A addition, etc. The product can be directly used for T/A vector cloning.
Activity Definition
1 unit (U) of Taq DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol of deoxynucleotides into acid-insoluble materials at 74°C for 30 minutes using activated salmon sperm DNA as a template primer.
Quality Control
1. The purity of SDS-PAGE is greater than 99%, and there is no exogenous nuclease activity after detection;
2. The PCR method detects no residual host DNA and can effectively amplify single-copy genes in the human genome
3. No significant activity change after storage at room temperature for one week
Transportation and storage methods
Transported with ice packs. Store at -20℃, valid for 2 years.
Precautions
1) For your safety and health, please wear a lab coat and disposable gloves when operating.
2) This product is for scientific research purposes only!
Recommended PCR reaction system (taking 50 μL reaction system as an example, prepared on ice)
Components | Volume (μL) |
ddH2O | to 50 |
10×Taq Buffer (with Mg 2+ ) | 5 |
dNTP Mix (10 mM each) | 1 μL |
Template DNA | ＜0.5 μg |
Primer 1 (10 μM) | 1 μL |
Primer 2 (10 μM) | 1 μL |
HRbio™ Taq DNA Polymerase (5 U/μL) | 0.5-1 μL |
【Note】: 1 ) Addition of polymerase: At room temperature, polymerase has a certain degree of 5'-3' polymerase activity. In order to prevent non-specific amplification, it is recommended to add polymerase to the reaction system in the last step.
2 ) Recommended usage of different templates ( 50 μL reaction system):
Template Type | Amplified fragment |
Genomic DNA | 50 ng～100 ng |
Plasmid DNA | 10 pg～20 ng |
cDNA | 1~5 μL (no more than 1/10 of the reaction system) |
PCR amplification procedure
Cycle steps | Temperature (C) | time | Number of cycles |
Pre-denaturation | 94 | 2-5min | 1 |
transsexual | 94 | 30 sec | 30 |
annealing | 50-60 | 30 sec | |
extend | 72 | 60 sec/1-2kb | |
Final extension | 72 | 5-10 min | 1 |
【Note】:
1) Pre-denaturation temperature and time: 94℃ is recommended. Pre-denaturation recommended time: 30 sec for simple templates such as plasmid DNA; 3 min for complex templates such as cDNA and genomic DNA; 5-10 min for templates with high GC content.
2) Annealing temperature and time: 60℃ is recommended. A temperature gradient can also be set up as needed to find the optimal temperature for index annealing. The recommended annealing time is set to 20 sec, which can be adjusted within 10-30 sec. Annealing time that is too long may cause the amplification product to be diffuse on the gel.
3) Amplification product: Please store the PCR amplification product at -20℃ to prevent DNA degradation.
