Product Information

Product Description

      EzRed™ Nucleic Acid Dye is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye that can replace the highly toxic ethidium bromide (EB). EzRed™ Nucleic Acid Dye is a unique oily macromolecule that cannot penetrate the cell membrane and enter the cell. It is not easy to volatilize and sublimate, and the human body will not inhale it. The results of the Ames test show that EzRed™ Nucleic Acid Dye is completely non-mutagenic at the gel staining concentration and is a safe and non-toxic nucleic acid dye. EzRed™ Nucleic Acid Dye has the same spectral characteristics as EB. There is no need to change the filter and observation device (ordinary UV gel transilluminator). It can be detected by ultraviolet light excitation at 300 nm. However, EzRed™ Nucleic Acid Dye cannot be excited by 488nm argon ion lasers or visible light of similar wavelengths. It is generally not recommended to use instruments with such excitation devices for imaging. EzRed™ Nucleic Acid Dye is suitable for dsDNA, ssDNA and RNA staining in agarose and polyacrylamide gel electrophoresis. You can choose gel staining or bubble staining for staining. It is very convenient and flexible to use. This product exists in the form of dissolving in aqueous solution. EzRed™ Nucleic Acid Stain dissolved in water is a safer formulation and is strongly recommended.

      EzRed™ Nucleic Acid Dye has the following features:

      1. Safer than EB: non-mutagenic and harmless to handle

      2. Much more sensitive than EB and SYBR™ Safe

      3. Stable at room temperature and can be heated in microwave

      4. Simple pre-cast or post-electrophoresis gel staining, no need for destaining

      5. Replacement of EB without changing equipment or optical setup

      6. Compatible with downstream gel purification, restriction digestion, sequencing and cloning

Transportation and storage

Transport at room temperature; store at room temperature away from light, valid for 5 years.

How to use

Since nucleic acid binding dyes can affect DNA migration, it is strongly recommended to perform electrophoresis first and then stain the gel post-staining. Post-staining with EzRed™ provides excellent sensitivity and eliminates the possibility of dye interference with DNA migration!!!

1. Gel staining (same as EB, staining before electrophoresis)

1. Prepare agarose gel of appropriate concentration and heat in a microwave oven until completely melted.

2. Add EzRed™ nucleic acid dye to a final concentration of 1× (i.e., add 5 μL of EzRed™ 10,000× aqueous solution to every 50 mL of agarose solution).

3. Pour the agarose solution containing EzRed™ nucleic acid dye into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.

4. Load the sample and perform electrophoresis as usual.

5. Take ultraviolet photos and observe.

【Note】: EzRed™ has good thermal stability and can be added directly to hot agarose solution. Alternatively, the EzRed™ stock solution can be added to the electrophoresis buffer containing agarose powder and then heated in a microwave or other common methods to prepare agarose gel. EzRed™ is compatible with all commonly used electrophoresis buffer solutions.

2. Foam dyeing method (staining after electrophoresis)

1. Prepare agarose gel of appropriate concentration and heat in a microwave oven until completely melted.

2. Pour the agarose solution into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.

3. Load the sample and perform electrophoresis as usual.

4. Dilute the EzRed™ 10,000× aqueous solution to 3× staining solution with 0.1 M NaCl solution (i.e., add 15 μL of EzRed™ 10,000× aqueous solution to 50 mL of 0.1 M NaCl solution. This staining solution can be reused about 3 times and should be stored at room temperature away from light).

5. Place the gel in a suitable container and add 3× staining solution to submerge the gel. Stain at room temperature for about 30 minutes with shaking. The optimal staining time is related to the thickness and concentration of the gel. For gels containing 3.5-10% polyacrylamide, the staining time is usually between 30 minutes and 1 hour, and is prolonged with the increase of polyacrylamide content.

6. Take ultraviolet photos and observe.

Precautions

1. If the macromolecular bands are tailing and the separation is not ideal, it is recommended to reduce the amount of DNA marker or nucleic acid sample loaded.

2. The gel staining method is not suitable for prefabricated polyacrylamide gels. For polyacrylamide gels, please use the bubble staining method.

3. For your safety and health, please wear lab coat and disposable gloves when operating.
4. This product is for scientific research purposes only!