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HRbio™ Rainbow Broad Spectrum Protein Marker (10-180kDa)
Price：
￥348
Cargo Number：
HRD0031
Specification：
250 μL 2×250 μL 10×250 μL
Specification and quality control：
Product Details
Product Specification
Product Information
Product Name | Product Number | Specification | Price (Yuan) |
HRbio™ Rainbow Broad Spectrum Protein Marker (10-180KD) | HRD0031 | 250 μL | 348.00 |
| HRD0032 | 2×250 μL | 616.00 | |
| HRD0033 | 10×250 μL | 2488.00 |
Product Description
This product is a mixture of 10 purified natural proteins ranging from 10 to 180 kD. The concentration of each band is about 0.2 to 0.4 mg/ml. The 75 kD band is red, the 25 kD band is green, and the remaining bands are blue. This product is suitable for molecular weight reference of denatured protein samples during SDS-PAGE electrophoresis, and can be used to observe the electrophoretic separation of protein samples in real time; it can also be used to detect the transfer efficiency of Western blot. Since covalently bound dyes affect the electrophoretic mobility of protein molecules,
this product can be used to roughly estimate the molecular weight of the target protein sample.
Instructions
1. After melting this product at room temperature, gently mix it to fully dissolve the precipitate.
2. Load 5uL, and clear rainbow bands will appear during SDS-PAGE electrophoresis and after transfer.
3. It is recommended to select the appropriate separation gel concentration based on the molecular weight of the protein to be tested.
Figure 1. Results of SDS-PAGE gel electrophoresis at different concentrations (Tris-Glycinet system)
Figure 2. Results of SDS-PAGE gel electrophoresis at different concentrations (Bis-Tris)
Transport with ice packs. Store at -20℃, valid for 2 years; store at 4℃, valid for 3 months.
Precautions
1) When using, the product should be restored to room temperature after being taken out from low temperature, mixed and then loaded; otherwise, the protein may not be completely denatured at low temperature, resulting in
different degrees of dispersion or tailing of the electrophoresis band.
2) The transfer effect is related to the transfer time, which depends on the size of the target band. If some of the larger molecular weight bands fail to transfer successfully after transfer, it is a normal
phenomenon.
3) This product contains SDS, the protein has been denatured, and it is not suitable as a molecular weight reference standard for electrophoresis of natural protein molecules.
4) This product should be avoided from repeated freezing and thawing. It is recommended to be stored in aliquots.
5) For your safety and health, please wear a lab coat and disposable gloves when operating.
6) This product is for scientific research purposes only!
