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4× protein loading buffer (containing sulfhydryl reducing agent)
Price：
￥14
Cargo Number：
HRD0124
Specification：
1 mL 10mL 25mL
Specification and quality control：
Product Details
Product Specification
Product Information
Product Name | Product Number | Specification | Price (Yuan) |
4×Protein Loading Buffer (with β-Me) 4× Protein Loading Buffer (Contains thiol reducing agent)
| HRD0124 | 1mL | 14.00 |
| HRD0125 | 10mL | 130.00 | |
| HRD0126 | 25mL | 300.00 |
Product Description
This product is suitable for protein loading in SDS-PAG E (SDS-denaturing polyacrylamide gel electrophoresis). Its main components are SDS, thiol reducing agent, bromophenol blue, buffered salt solution, etc. SDS can bind to protein to make the protein-SDS complex carry a large amount of negative charge. At this time, the charge of the protein itself is completely masked by SDS, eliminating the difference in the charge of various proteins themselves; SDS can also break the hydrogen bonds within and between molecules, destroying the secondary and tertiary structures of protein molecules. The thiol reducing agent can break the disulfide bonds between cysteine residues, destroy the protein structure, and eliminate the differences between protein structures. In the end, the electrophoresis speed of proteins (subunits) with no charge and structural differences is only related to their molecular weight. Bromophenol blue is used as an indicator during electrophoresis to roughly indicate the time when electrophoresis ends.
Instructions for use:
1. Please use the ratio of 10 μL loading buffer for every 30 μL protein sample (4-fold dilution). If the protein sample concentration is too high, dilute it with double distilled water.
2. After mixing, heat in a 100°C water bath for 5-10 minutes to denature the protein.
3. After cooling to room temperature, centrifuge at 10000-14000rpm for 2-5 minutes, take the supernatant and directly load it for electrophoresis.
Transportation and storage methods
Transport with ice packs. Store at -20℃ away from light.
Precautions
1. This reagent contains thiol reducing agent, which is toxic. For your safety and health, please wear lab coat and disposable gloves when operating.
2. This product is for scientific research purposes only!
3. When the concentration of polyacrylamide gel is 8%, the position of the bromophenol blue indicator band is about 30kd, when the concentration is 12%, it is about 20kd, and when the concentration is 15%, it is about 10kd. Please judge the electrophoresis time according to your target band.
4. The protein loading buffer contains bromophenol blue indicator. The pH value is affected by the storage temperature. When stored at low temperature, the solution may appear dark brown, which does not affect the use of the product.
