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Commonly used antibiotic solutions
Time：2024-09-12
| Storage solution a | Working concentration | ||
concentration | Storage conditions | Stringent plasmid | Relaxed plasmid | |
| Ampicillin | 50mg/ml(soluble in water) | -20℃ | 20μg/ml | 60μg/ml |
| Carbenicillin | 50mg/ml(soluble in water) | -20℃ | 20μg/ml | 60μg/ml |
| Chloramphenicol | 34mg/ml (soluble in ethanol) | -20℃ | 25μg/ml | 170μg/ml |
| Kanamycin | 10mg/ml(soluble in water) | -20℃ | 10μg/ml | 50μg/ml |
| Streptomycin | 10mg/ml(soluble in water) | -20℃ | 10μg/ml | 50μg/ml |
| Tetracycline b | 5mg/ml (soluble in ethanol) | -20℃ | 10μg/ml | 50μg/ml |
a: Antibiotic stock solutions with water as solvent should be sterilized by filtration through a 0.22μm filter. Antibiotic solutions with ethanol as solvent do not need to be sterilized. All antibiotic solutions should be stored in light-proof containers.
b: Magnesium ions are an antagonist of tetracycline. Screening for tetracycline-resistant bacteria should use a culture medium that does not contain magnesium salts (such as LB culture medium).
